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Activity of DNA modification and restriction enzymes in KGB, a potassium glutamate buffer
1Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.
Gene Analysis Techniques
|September 1, 1988
Abstract:
A single buffer consisting primarily of potassium and glutamate is shown to be suitable for activity of almost all restriction endonucleases, DNA methylases, and many other DNA-modifying enzymes. The composition of this buffer differs substantially from buffers currently in use for these enzymes.