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Updated: Feb 20, 2026

Conducting Maximal and Submaximal Endurance Exercise Testing to Measure Physiological and Biological Responses to Acute Exercise in Humans
Published on: October 17, 2018
Influence of exercise duration on cardiorespiratory responses, energy cost and tissue oxygenation within a 6 hour
Hugo A Kerhervé1, Scott McLean1, Karen Birkenhead1
1School of Health and Sport Sciences, University of the Sunshine Coast, Sippy Downs, QLD, Australia.
Purpose:
The physiological mechanisms for alterations in oxygen utilization ([Formula: see text]) and the energy cost of running (C ) during prolonged running are not completely understood, and could be linked with alterations in muscle and cerebral tissue oxygenation.
Methods:
Eight trained ultramarathon runners (three women; mean ± SD; age 37 ± 7 yr; maximum [Formula: see text] 60 ± 15 mL min-1 kg-1) completed a 6 hr treadmill run (6TR), which consisted of four modules, including periods of moderate (3 min at 10 km h-1, 10-CR) and heavy exercise intensities (6 min at 70% of maximum [Formula: see text], HILL), separated by three, 100 min periods of self-paced running (SP). We measured [Formula: see text], minute ventilation ([Formula: see text]), ventilatory efficiency ([Formula: see text]), respiratory exchange ratio (RER), C , muscle and cerebral tissue saturation index (TSI) during the modules, and heart rate (HR) and perceived exertion (RPE) during the modules and SP.
Results:
Participants ran 58.3 ± 10.5 km during 6TR. Speed decreased and HR and RPE increased during SP. Across the modules, HR and [Formula: see text] increased (10-CR), and RER decreased (10-CR and HILL). There were no significant changes in [Formula: see text], [Formula: see text], C , TSI and RPE across the modules.
Conclusions:
In the context of positive pacing (decreasing speed), increased cardiac drift and perceived exertion over the 6TR, we observed increased RER and increased HR at moderate and heavy exercise intensity, increased [Formula: see text] at moderate intensity, and no effect of exercise duration on ventilatory efficiency, energy cost of running and tissue oxygenation.
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