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Brachypodium distachyon Long Noncoding RNAs: Genome-Wide Identification and Expression Analysis
Concetta De Quattro1, Erica Mica2, Mario Enrico Pè1
1Institute of Life Sciences, Scuola Superiore Sant'Anna, Piazza Martiri della Libertà 33, 56127, Pisa, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|October 18, 2017
Summary
Researchers developed an efficient method to identify long noncoding RNAs (lncRNAs) using next-generation sequencing. This advancement aids in understanding the complex roles of lncRNAs in biological processes, especially in plants.
Area of Science:
- Genomics
- Molecular Biology
- Bioinformatics
Background:
- High-throughput sequencing reveals extensive transcriptional activity in eukaryotic genomes.
- Noncoding RNAs (ncRNAs), including long ncRNAs (lncRNAs), play crucial roles in biological processes.
- The functions and regulatory mechanisms of lncRNAs, particularly in plants, are not well understood.
Purpose of the Study:
- To describe an efficient methodology for identifying long noncoding RNAs.
- To facilitate the characterization of lncRNAs using next-generation sequencing data.
Main Methods:
- Utilized next-generation sequencing data.
- Developed an efficient identification methodology for lncRNAs.
Main Results:
- Successfully identified long noncoding RNAs.
- Provided a method applicable to plant systems.
Conclusions:
- The described methodology enables efficient identification of lncRNAs.
- This work contributes to exploring the largely unexplored regulatory roles of lncRNAs in plants.