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Related Experiment Videos

Developments in Hematopoietic Stem Cell Expansion and Gene Editing Technologies.

Dogacan Yucel1, Fatih Kocabas2,3

  • 1Faculty of Medicine, University of Minnesota, Minneapolis, MN, USA. dyucel@umn.edu.

Advances in Experimental Medicine and Biology
|October 18, 2017
PubMed
Summary

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Hematopoietic stem cells (HSCs) expansion and gene editing are crucial for treating blood disorders. This review highlights advancements in technologies to overcome challenges like spontaneous differentiation during ex vivo HSC procedures.

Area of Science:

  • * Hematopoietic stem cell biology and regenerative medicine.

Background:

  • * Hematopoietic stem cells (HSCs) reside in bone marrow and sustain blood cell production.
  • * Limited HSC availability for transplantation necessitates improved expansion and gene editing techniques.
  • * Spontaneous differentiation and cost-efficiency are major hurdles in ex vivo HSC expansion.

Purpose of the Study:

  • * To review recent developments in hematopoietic stem cell expansion technologies.
  • * To highlight advancements in gene editing applications for HSCs.
  • * To discuss strategies for overcoming ex vivo expansion challenges.

Main Methods:

  • * Review of existing literature on HSC expansion using cytokines, growth factors, small molecules, and RNA interference.
  • * Analysis of gene editing technologies applied to HSCs.
Keywords:
Cell cycle progressionGene editingHSC expansionSingle cell expansionSmall molecules

Related Experiment Videos

  • * Examination of methods to enhance cell cycle progression and block inhibitory signaling.

    • Main Results:

    • * Identification of small molecules and RNA interference as promising tools for HSC expansion.
    • * Progress in gene editing for correcting genetic defects in HSCs.
    • * Strategies to mitigate spontaneous differentiation during ex vivo culture.

    Conclusions:

    • * Significant progress has been made in HSC expansion and gene editing technologies.
    • * Further research is needed to optimize ex vivo procedures for clinical applications.
    • * These advancements hold promise for treating inherited bone marrow disorders and anemia.