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Updated: Feb 20, 2026

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
Published on: July 6, 2019
Rapid nonlinear image scanning microscopy
Ingo Gregor1,2, Martin Spiecker1, Roman Petrovsky3
13rd Institute of Physics - Biophysics, University of Göttingen, Göttingen, Germany.
Abstract:
Image scanning microscopy (ISM) doubles the resolution of a conventional confocal microscope for super-resolution imaging. Here, we describe an all-optical ISM design based on rescanning microscopy for two-photon-excited fluorescence and second-harmonic generation that allows straightforward implementation into existing microscopes. The design offers improved sensitivity and high frame rates relative to those of existing systems. We demonstrate its utility using fixed and living specimens as well as collagen hydrogels.
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