Related Experiment Videos
Transglutaminase in cell proliferation and transformation
S Metafora1, G Peluso, G Ravagnan
1CNR Institute of Protein Biochemistry and Enzymology, Naples, Italy.
Medical Oncology and Tumor Pharmacotherapy
|January 1, 1988
Summary
Transglutaminase (TGase) activity decreases in stimulated lymphocytes and transformed cells, suggesting its role in cell proliferation and cancer.
Area of Science:
- Biochemistry
- Cell Biology
- Oncology
Background:
- Transglutaminase (TGase) is an enzyme involved in various cellular processes.
- Its role in cell proliferation and transformation requires further investigation.
Purpose of the Study:
- To investigate the role of TGase activity in human peripheral blood lymphocytes (PBL) and neoplastic cells.
- To determine if TGase can modulate mitogen-induced lymphocyte activation.
Main Methods:
- Assessed TGase activity in resting and mitogen-stimulated human PBL.
- Treated quiescent PBL with purified liver TGase and Ca2+ to observe effects on blast transformation.
- Compared TGase activity in normal and neoplastically transformed seminal vesicle epithelial cells.
Main Results:
- TGase activity was lower in mitogen-stimulated PBL compared to resting PBL.
- Exogenous TGase and Ca2+ suppressed mitogen-induced blast transformation in PBL.
- Neoplastically transformed seminal vesicle epithelial cells exhibited decreased TGase activity compared to normal cells.
Conclusions:
- TGase activity appears to be inversely correlated with cell proliferation.
- TGase may play a role in regulating lymphocyte activation.
- Reduced TGase activity is observed in neoplastic transformation, suggesting its potential involvement in cancer development.