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Updated: Feb 20, 2026

A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice
Published on: April 16, 2018
[Corrigendum] Farnesoid X receptor deletion improves cardiac function, structure and remodeling following myocardial
Jianshu Gao1, Xiaoqiang Liu2, Bingjian Wang3
1Department of Cardiology, Shanghai General Hospital of Nanjing Medical University, Shanghai 200080, P.R. China.
Abstract:
Following the publication of this article, an interested reader drew to our attention that we had incorrectly reported (in the Materials and methods section, 'Western blot analysis', on p. 674) that the anti-farnesoid X receptor (FXR) antibody of Cell Signalling Technology, Inc., cat. no. #12295, had been used in this study to probe for FXR. In fact, the antibodies used in the above-mentioned study were a gift from the group of Dr Xin-Liang Ma at Thomas Jefferson University (Philadelphia, PA, USA), as referenced in the following article: [Pul J, Yuan A, Shan P, Gao E, Wang X, Wang Y, Lau WB, Koch W, Xin-Ma XL and He B: Cardiomyocyte-expressed farnesoid-X-receptor is a novel apoptosis mediator and contributes to myocardial ischaemia/reperfusion injury. Eur Heart J 34: 1834-1845, 2013]. The antibody that was used for the western blotting analysis was raised against the C-terminus of FXR (C-20; cat. no. sc-1204, Santa Cruz Biotechnology, San Diego, CA, USA). We sincerely apologize for this mistake, and thank the reader of our article who drew this matter to our attention. The error made in our incorrect referencing of the antibody did not affect the conclusions reported in this study. Furthermore, we regret the inconvenience that this mistake caused. [the original article was published in the Molecular Medicine Reports 16: 673-679, 2017; DOI: 10.3892/mmr.2017.6643].
Insights
A correction clarifies the specific antibody used for farnesoid-X receptor (FXR) western blot analysis in a study on myocardial ischemia/reperfusion injury. The findings remain valid despite the methodological error.
Area of Science:
- Cardiovascular Research
- Molecular Biology
- Biochemistry
Background:
- The study investigated the role of farnesoid-X receptor (FXR) in myocardial ischemia/reperfusion injury.
- Accurate methodological details are crucial for reproducibility and scientific integrity.
Purpose of the Study:
- To correct a methodological error regarding the antibody used for western blot analysis of FXR.
- To confirm that the scientific conclusions of the original study remain unaffected by this correction.
Main Methods:
- Western blot analysis was performed to detect FXR.
- A specific antibody against the C-terminus of FXR (Santa Cruz Biotechnology, sc-1204) was used.
- The correction clarifies the source and specifications of the antibody used.
Main Results:
- The original study incorrectly cited an antibody from Cell Signalling Technology.
- The correct antibody used was from Santa Cruz Biotechnology, raised against the C-terminus of FXR.
- The error in antibody referencing did not alter the study's main conclusions.
Conclusions:
- The scientific findings regarding FXR's role in myocardial ischemia/reperfusion injury are robust.
- This correction ensures accurate reporting of experimental methods.
- Transparency in scientific reporting is essential for the research community.
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