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Cell type-dependent localization of MLO proteins.

Daniel S Jones1, Sharon A Kessler2,3

  • 1a Department of Microbiology and Plant Biology , The University of Oklahoma , Norman , OK , USA.

Plant Signaling & Behavior
|October 18, 2017
PubMed
Summary

Mildew resistance locus O (MLO) proteins

Keywords:
Cell biologyGolgiMLOendomembrane systemgolgi apparatusplant sexual reproductionsecretory pathwaysecretory systemsubcellular localizationtransient expression

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Area of Science:

  • Plant molecular biology
  • Cell biology
  • Genetics

Background:

  • Mildew resistance locus O (MLO) proteins are transmembrane proteins crucial for plant cell-cell communication.
  • Subcellular localization of MLO proteins, like NORTIA (NTA), is vital for pollen tube reception.
  • NTA localizes to the Golgi in synergid cells during pollen tube interaction.

Purpose of the Study:

  • To investigate the cell type-dependency of MLO protein subcellular localization.
  • To understand how different cellular environments affect MLO distribution and function.
  • To identify potential co-factors influencing MLO localization.

Main Methods:

  • Ectopic expression of MLO proteins in different plant cell types (tobacco and Arabidopsis epidermal cells vs. synergid cells).
  • Confocal microscopy to visualize MLO protein localization using Golgi markers.
  • Comparative analysis of MLO distribution across distinct cell types.

Main Results:

  • MLO protein subcellular localization is dependent on the cell type.
  • Distinct MLO distributions were observed when ectopically expressed in epidermal cells compared to synergid cells.
  • Some MLOs that functionally substitute for NTA showed partial co-localization with Golgi markers in synergid cells.

Conclusions:

  • Cell type-specific factors likely influence MLO protein distribution and function.
  • Understanding MLO localization provides new targets for studying their roles in various plant processes.
  • This cell type-dependency offers insights into the regulation of MLO protein activity.