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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Updated: Feb 20, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
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Single lens logarithmic confocal distance measurement array.

Jin Wang

    Optics Express
    |October 19, 2017
    PubMed
    Summary

    This study introduces a confocal microscopy method for precise, non-contact distance measurements to reflective surfaces. The technique uses photodetector signal ratios, independent of reflectivity, for accurate object distance determination.

    Area of Science:

    • Optical Metrology
    • Microscopy Techniques
    • Surface Science

    Background:

    • Accurate non-contact distance measurement is crucial in various scientific fields.
    • Existing methods can be limited by surface reflectivity and light source variations.

    Purpose of the Study:

    • To develop a novel confocal microscopy-based method for axial distance measurement.
    • To achieve measurements independent of surface reflectivity and light source fluctuations.

    Main Methods:

    • Utilized confocal microscopy with an array of photodetectors.
    • Analyzed the ratio of photodetector signals.
    • Employed logarithmic transformation of photodetector signals.

    Main Results:

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  • Established a method for non-contact axial distance measurement.
  • Demonstrated that the signal ratio is independent of surface reflectivity and light source variations.
  • Showed object distance is proportional to the difference between logarithmic photodetector signals.

    • Conclusions:

    • The developed technique offers a robust solution for distance measurement.
    • Potential applications include measuring cellular acoustics and laser fusion dynamics.