Investigating long-term effect of nanoparticles on growth of Raphanus sativus plants: a trans-generational study

In the past decade, there has been an unprecedented growth in the application of nanoparticles (NPs) worldwide. Even though the acute toxicity of CuO and ZnO NPs to plants has been investigated in past, the trans-generational effects of these NPs in the environment are still unknown. In this study, we investigated whether the treatment of radish plants with CuO and ZnO NPs as single compound and as a binary mixture (10, 100 and 1000 mg/Kg soil) through their lifecycle would affect the seed quality and the development of second-generation seedlings or not. Results showed reduced root length, shoot length and biomass in F1 seedlings of NPs treated plants. The treated F1 seeds had smaller seed weight with accumulated Cu and Zn. The effect of toxic interaction between CuO and ZnO on plant growth was antagonistic in nature. Evaluation of the trans-generational impact is important to understand the long-term effect of NPs on the environment.