Related Experiment Video
Updated: Aug 7, 2026

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
Published on: May 24, 2017
Study of UV-induced DNA Repair Factor Recruitment: Kinetics and Dynamics
Sarah Sertic1, Stefania Roma1, Paolo Plevani1
1Dipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, Milano, 20133, Italy.
Abstract:
The local UV irradiation technique enables detection, kinetic measurements of recruitment, and quantification of DNA Damage Response (DDR) proteins at the site of UV-induced DNA damage.Using Isopore filters with high density pores of a broad range of sizes, it is possible to UV irradiate and damage only a very small portion of the nucleus of a cell by letting UV light pass only through the pores. Immunofluorescent analyses of modified DNA nucleotides, proteins, or fluorescently tagged versions of target factors can be used as markers to label and study UV-induced lesions and their repair.
More Related Videos
Related Concept Videos
Nucleotide Excision Repair
Overview of DNA Repair
Chemically...
Long-patch Base Excision Repair
Nucleotide Excision Repair
Overview of DNA Repair
Chemically...
Nucleotide Excision Repair
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...

