Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Next-Generation Sequencing in Differentiated Thyroid Cancer Patients Treated with Lenvatinib: Results and Challenges in Real-Life Practice.

Current oncology (Toronto, Ont.)·2026
Same author

Progression-directed ablative radiotherapy improves event-free survival in oligoprogressive NSCLC.

Frontiers in oncology·2026
Same author

Biomass and coccolith productions in Chrysotila sp. in high density cultivation system.

Applied microbiology and biotechnology·2026
Same author

A phase I, needle free, dose escalation clinical trial of pEVAC-PS, a candidate pan-Sarbecovirus Vaccine.

The Journal of infection·2026
Same author

Inf-Sup Stable Space-Time Discretization of the Wave Equation Based on a First-Order-In-Time Variational Formulation.

Journal of scientific computing·2026
Same author

Impact of Tau overexpression on DNA replication dynamics in centromeres of human neural progenitor cells.

iScience·2026

Related Experiment Video

Updated: Jun 17, 2026

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells
11:58

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells

Published on: March 5, 2018

A qPCR-Based Protocol to Quantify DSB Resection.

Matteo Ferrari1, Shyam Twayana1, Federica Marini1

  • 1Dipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133, Milano, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|October 19, 2017
PubMed
Summary
This summary is machine-generated.

DNA end resection, a crucial step in double-strand DNA break repair, is quantified using a novel qPCR protocol. This method accurately measures resection rates, aiding in understanding genome stability across organisms.

Keywords:
DNA end resectionDouble-strand breaksSingle-strand DNAqPCR

More Related Videos

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
07:54

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

Related Experiment Videos

Last Updated: Jun 17, 2026

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells
11:58

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells

Published on: March 5, 2018

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
07:54

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

Area of Science:

  • Molecular Biology
  • Genetics
  • DNA Repair Mechanisms

Background:

  • Homologous recombination is essential for accurate repair of double-strand DNA breaks (DSBs).
  • Initiation of homologous recombination requires nucleolytic degradation of the 5'-ending strand at the break site, a process known as DNA end resection.
  • Precise regulation of DNA end resection is critical to prevent genomic instability and rearrangements.

Purpose of the Study:

  • To describe a quantitative method for assessing the rate of DNA end resection at double-strand DNA breaks.
  • To provide a versatile protocol applicable to various organisms for studying DNA repair dynamics.

Main Methods:

  • Development of a quantitative Polymerase Chain Reaction (qPCR) based assay.
  • The protocol quantifies the extent of DNA end resection at a known break site.
  • Requires knowledge of the DNA sequences flanking the break site for primer design.

Main Results:

  • Successfully established a qPCR-based protocol to measure DSB resection rates.
  • The method provides a quantifiable metric for the efficiency of DNA end resection.
  • Demonstrated the potential applicability across different organisms.

Conclusions:

  • The described qPCR protocol offers a reliable method to quantify DNA end resection rates.
  • This technique facilitates the study of DNA repair mechanisms and genome stability.
  • The protocol's broad applicability makes it a valuable tool in diverse biological research.