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Quantitative Analysis of the Rates for Repeat-Mediated Genome Instability in a Yeast Experimental System
Elina A Radchenko1, Ryan J McGinty1, Anna Y Aksenova2
1Department of Biology, Tufts University, 200 Boston Avenue, Medford, MA, 02155, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 19, 2017
Abstract:
Instability of repetitive DNA sequences causes numerous hereditary disorders in humans, the majority of which are associated with trinucleotide repeat expansions. Here, we describe a unique system to study instability of triplet repeats in a yeast experimental setting. Using fluctuation assay and the novel program FluCalc we are able to accurately estimate the rates of large-scale expansions, as well as repeat-mediated mutagenesis and gross chromosomal rearrangements for different repeat sequences.