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Imaging of DNA Ultrafine Bridges in Budding Yeast
Oliver Quevedo1,2, Michael Lisby3,4
1Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200, Copenhagen N, Denmark.
Methods in Molecular Biology (Clifton, N.J.)
|October 19, 2017
Abstract:
DNA ultrafine bridges (UFBs) are a type of chromatin-free DNA bridges that connect sister chromatids in anaphase and pose a threat to genome stability. However, little is known about the origin of these structures, and how they are sensed and resolved by the cell. In this chapter, we review tools and methods for studying UFBs by fluorescence microscopy including chemical and genetic approaches to induce UFBs in the model organism Saccharomyces cerevisiae.