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Related Experiment Videos

Rewiring the Budding Yeast Proteome using Synthetic Physical Interactions.

Guðjón Ólafsson1, Peter H Thorpe2

  • 1Mitotic Control Laboratory, The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK.

Methods in Molecular Biology (Clifton, N.J.)
|October 19, 2017
PubMed
Summary

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We developed a new method to force proteins to interact, enabling large-scale screening of protein functions. This approach helps discover new molecular mechanisms by observing how forced protein associations affect cell growth and drug sensitivity.

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Systems Biology

Background:

  • Protein interactions are crucial for cellular functions.
  • Current methods for studying protein interactions are limited in scale and require prior knowledge.
  • Investigating novel protein associations can reveal new biological insights.

Purpose of the Study:

  • To develop a scalable method for creating and screening artificial protein-protein interactions.
  • To enable proteome-wide discovery of functional protein associations.
  • To identify protein interactions affecting cell growth and drug responses.

Main Methods:

  • Developed the Synthetic Physical Interaction (SPI) method.
  • Applied SPI in Saccharomyces cerevisiae (budding yeast).
Keywords:
ChromobodyGFP-binding protein (GBP)Green fluorescent protein (GFP)High-throughput screenNanobodyProtein-protein interactions (PPI)Selective ploidy ablation

Related Experiment Videos

  • Screened thousands of forced protein associations for functional effects.

    • Main Results:

    • The SPI method allows for proteome-wide screening of forced protein associations.
    • Identified protein associations that impact normal cell growth.
    • Discovered protein associations that alter sensitivity to drugs and specific conditions.

    Conclusions:

    • The SPI method provides a powerful, unbiased approach to discover functional protein-protein interactions.
    • This technique facilitates large-scale screening for novel molecular mechanisms.
    • SPI is applicable to various genetic elements, including proteins, domains, and peptides.