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Related Experiment Videos

Labeling the ER for Light and Fluorescence Microscopy.

Chris Hawes1, Pengwei Wang2, Verena Kriechbaumer3

  • 1Department of Biological and Medical Sciences, Oxford Brookes University, OX3 0BP, Oxford, UK. chawes@brookes.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|October 19, 2017
PubMed
Summary

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This study presents methods for labeling the endoplasmic reticulum (ER) in its native state using fluorescent proteins, lipid dyes, and immunolabeling techniques in plant tissues for improved imaging.

Area of Science:

  • Cell Biology
  • Plant Science
  • Biochemistry

Background:

  • The endoplasmic reticulum (ER) is a dynamic organelle crucial for cellular function.
  • Imaging the ER in its native, mobile state is essential for understanding its dynamic behavior.
  • Existing imaging methods may not fully capture the native state of the ER.

Purpose of the Study:

  • To develop and describe effective methods for labeling the native endoplasmic reticulum (ER).
  • To enable advanced imaging of the ER in its natural, mobile condition.
  • To provide techniques applicable to plant tissues.

Main Methods:

  • Utilized fluorescent proteins for ER labeling.
  • Employed lipid dyes for visualizing the ER membrane.
  • Developed immunolabeling protocols for plant tissues.
Keywords:
Endoplasmic reticulumFluorescent dyesFluorescent proteinImmunofluorescenceLabelingStable expressionTransient expression

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Main Results:

  • Successfully labeled the native ER in plant cells using the described methods.
  • Demonstrated the capability to image the dynamic ER network.
  • Established robust immunolabeling techniques for ER studies in plants.

Conclusions:

  • The described methods provide powerful tools for studying the native endoplasmic reticulum in plants.
  • Advanced imaging of the dynamic ER is now more accessible.
  • These techniques will facilitate deeper understanding of ER structure and function in vivo.