Related Experiment Video
Updated: Oct 2, 2026
Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry
Published on: December 28, 2021
Gene Polymorphism of Matrix Metalloproteinase 9 in Asthenozoospermic Male Subjects
Sina Mohagheghi1, Iraj Khodadadi2, Manoochehr Karami3
1Student Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Background:
Matrix metalloproteinase (MMPs) play important roles in the structural and functional properties of reproductive organs. The aim of this study is to determine the prevalence of C-1562T MMP-9 (rs3918242) gene polymorphism in fertile and infertile men. In addition, we aim to determine the association between C-1562T MMP-9 and G-1575A MMP-2 gene polymorphisms.
Materials And Methods:
A total of 400 subjects, including 200 fertile and 200 infertile men, were recruited for this casecontrol study. The allele frequencies and genotype distributions of single nucleotide polymorphism in the promoter regions of MMP-9 (C-1562T) were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The chi-square (χ2) test was used to assess the distribution of genotype frequencies.
Results:
There were no significant differences found in the genotype distributions or allele frequencies between fertile and infertile men for the C-1562T MMP-9 gene polymorphism. The percent of immotile sperm in infertile men with the CC and CT genotypes of C-1562T MMP-9 gene polymorphism significantly differed compared with that of subjects with the TT genotype. The frequency of CC/GA-combined genotypes of C-1562T MMP-9 and G-1575A MMP-2 gene polymorphisms significantly differed in fertile and infertile men (P=0.031).
Conclusion:
Our results suggest that genetic polymorphisms in MMP may impact male fertility.
Insights
Genetic variations in matrix metalloproteinase (MMP) genes may influence male fertility. This study investigated MMP-9 and MMP-2 gene polymorphisms in fertile and infertile men, finding potential associations with sperm motility and fertility status.
Area of Science:
- Reproductive biology
- Human genetics
- Molecular biology
Background:
- Matrix metalloproteinases (MMPs) are crucial for reproductive organ structure and function.
- Genetic variations in MMP genes are being investigated for their role in reproductive health.
Purpose of the Study:
- To determine the prevalence of C-1562T MMP-9 gene polymorphism in fertile and infertile men.
- To investigate the association between C-1562T MMP-9 and G-1575A MMP-2 gene polymorphisms with male infertility.
Main Methods:
- Case-control study involving 400 men (200 fertile, 200 infertile).
- Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) used to analyze MMP-9 (C-1562T) and MMP-2 (G-1575A) gene polymorphisms.
- Chi-square test applied to assess genotype and allele frequency distributions.
Main Results:
- No significant differences in genotype or allele frequencies for C-1562T MMP-9 polymorphism between fertile and infertile groups.
- Significant differences in immotile sperm percentage observed for C-1562T MMP-9 genotypes (CC/CT vs. TT) in infertile men.
- Combined CC/GA genotypes of MMP-9 and MMP-2 showed a significant difference in frequency between fertile and infertile men (P=0.031).
Conclusions:
- Genetic polymorphisms in MMP genes may impact male fertility.
- Specific MMP gene variations are associated with sperm immotility and fertility status in men.
Related Concept Videos
01:41Spermatogenesis
01:23Infertility in Males