Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Composite Hydrogels Containing Bioactive Microreactors for Optical Enzymatic Lactate Sensing.

Aniket Biswas1, Lindsey R Bornhoeft1, Swayoma Banerjee1

  • 1Department of Biomedical Engineering, ‡Department of Biology, and §Department of Materials Science and Engineering, Texas A&M University , College Station, Texas 77843, United States.

ACS Sensors
|October 19, 2017
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Granular Hydrogel Composites for Noninvasive Optical Biosensing.

Journal of biomedical materials research. Part A·2026
Same author

Development of an Insertable Enzymatic Sensor Towards <i>in vivo</i> Optical Measurements of Amino Acids.

IEEE sensors letters·2026
Same author

Smart Catheters for Diagnosis, Monitoring, and Therapy.

Advanced healthcare materials·2025
Same author

NIR Phosphorescent Oxygen Sensors in Natural Hydrogel Matrices.

bioRxiv : the preprint server for biology·2025
Same author

Phosphorescence Lifetime Imaging for Monitoring an Insertable Hydrogel Multianalyte Sensor.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same author

Insertable Biomaterial-Based Multianalyte Barcode Sensor toward Continuous Monitoring of Glucose and Oxygen.

ACS sensors·2024

This study presents a novel composite hydrogel sensor for optical lactate detection. The stable, implantable sensor shows potential for continuous disease monitoring.

Area of Science:

  • Biomaterials Science
  • Chemical Sensors
  • Optical Biosensing

Background:

  • Continuous monitoring of biomarkers like lactate is crucial for understanding disease status and progression.
  • Existing methods for lactate detection may have limitations in continuous monitoring or implantability.

Purpose of the Study:

  • To develop and characterize a composite hydrogel-based sensing platform for optical detection of lactate.
  • To evaluate the sensor's performance, stability, and potential for continuous, implantable monitoring.

Main Methods:

  • Fabrication of a composite hydrogel embedding microsized enzymatic sensors.
  • Encapsulation of lactate oxidase as the bioactive component and phosphorescent metalloporphyrin as the optical transducer.
  • Utilizing polyelectrolyte multilayers to control lactate permeation and characterizing sensor response under physiological conditions.
Keywords:
alginatebiosensorcomposite hydrogellactate sensinglayer-by-layer self-assemblyphosphorescence

Related Experiment Videos

Main Results:

  • The lactate sensors demonstrated an analytical range of 9.2 ± 0.83 mg/dL and a mean sensitivity of 11 ± 0.90% dL mg^-1.
  • The sensors exhibited excellent stability, with no significant loss of response after 20 cycles of high lactate exposure.
  • Performance was validated under low physiological oxygen levels.

Conclusions:

  • The developed composite hydrogel platform is capable of optical lactate detection.
  • The sensors show high stability and sensitivity, indicating potential for continuous lactate tracking.
  • These findings support the development of fully implantable optical lactate sensors for disease management.