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Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
Published on: April 11, 2016
Pectobacterium atrosepticum exopolysaccharides: identification, molecular structure, formation under stress and in
Vladimir Gorshkov1,2, Bakhtiyar Islamov1,2, Polina Mikshina1
1Kazan Institute of Biochemistry and Biophysics, Kazan Science Centre, Russian Academy of Sciences, Lobachevsky Str. 2/31, P.O. Box 30, 420111 Kazan, Russia.
Abstract:
In the present study, we identified exopolysaccharides of the harmful phytopathogenic bacterium Pectobacterium atrosepticum SCRI1043 and characterized the molecular structure of these polymers. The synthesis of the target polysaccharides was shown to be induced under starvation conditions. Moreover, intensive accumulation of exopolysaccharides occurred during the colonization by bacteria of the xylem vessels of infected plants, where microorganisms formed specific 3D "multicellular" structures-bacterial emboli. Thus, the identified polymers are likely to be involved in the adaptation and virulence of bacteria of Pectobacterium genus.
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