Targeted proteomic analysis of habitual coffee consumption.

Area of Science:

• Biomedical science
• Epidemiology
• Proteomics

Background:

• Coffee consumption's impact on mortality and disease is known, but underlying mechanisms remain unclear.
• Large-scale epidemiological studies can provide insights into coffee's health associations.

Purpose of the Study:

• To investigate the association between habitual coffee intake and cardiovascular disease-related protein markers.
• To identify novel protein markers associated with coffee consumption.

Main Methods:

• Analysis of up to 80 plasma protein markers using a proximity extension assay.
• Utilized data from three large cohorts: PIVUS, ULSAM, and EpiHealth.
• Coffee intake assessed via dietary records and food frequency questionnaires.

Main Results:

• Identified significant associations between coffee intake and leptin (LEP), chitinase-3-like protein 1 (CHI3L1), TNF receptor 6, and TNF-related apoptosis-inducing ligand in the PIVUS cohort.
• The inverse association between coffee intake and LEP was replicated in ULSAM and EpiHealth cohorts.
• A novel inverse association between coffee intake and CHI3L1 was observed, replicating in EpiHealth.

Conclusions:

• Coffee intake is inversely associated with plasma levels of leptin and CHI3L1.
• The novel association between coffee and CHI3L1 warrants further research due to CHI3L1's links to various health conditions.
• Findings contribute to understanding coffee's physiological effects and potential health impacts.