Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanoadaptive root growth in <i>Medicago sativa</i> under controlled microhydrodynamic environments.

Lab on a chip·2026
Same author

A Swivel Microwell Platform for Rotational Assembly and Co-culture of Multiple Spheroids.

Biofabrication·2026
Same author

Wrong-side imaging orders: automated detection using electronic health record data - a retrospective cohort study.

BMJ open quality·2026
Same author

Population-Specific Genetic and Non-Genetic Influences on Sleep Traits and Health Outcomes.

ArXiv·2026
Same author

Yeast-powered microfluidic pump based on a four-parameter fermentation model.

Microsystems & nanoengineering·2026
Same author

Toward scalable early cancer detection: evaluating EHR-based predictive models against traditional screening criteria.

NPJ precision oncology·2026
Same journal

Inorganic biomaterials-reinforced printable hydrogel modulating regenerative microenvironments for tissue repair.

Biofabrication·2026
Same journal

Modeling respiratory viral infections and investigating immune responses: new advances in human organ chip models.

Biofabrication·2026
Same journal

Floatony formation in liquid environments: liquid drawing-based fabrication of three-dimensional microbial structures.

Biofabrication·2026
Same journal

Magneto-Archimedes based 3D cell economic bioassembly.

Biofabrication·2026
Same journal

Open-air human skin equivalent platform enabling photobiological studies and topical product testing.

Biofabrication·2026
Same journal

Engineering the esophagus: advances, challenges, and translational pathways in esophageal tissue reconstruction.

Biofabrication·2026
See all related articles

Related Experiment Video

Updated: Feb 20, 2026

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids
10:27

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Published on: November 7, 2019

6.9K

Hypergravity-induced multicellular spheroid generation with different morphological patterns precisely controlled on

Jiheum Park1, Gi-Hun Lee, Joong Yull Park

  • 1Interdisciplinary Program for Bioengineering, Seoul National University Graduate School, Seoul 03087, Republic of Korea.

Biofabrication
|October 19, 2017
PubMed
Summary
This summary is machine-generated.

A new centrifugal microfluidic-based spheroid (CMS) method creates uniform 3D cell cultures. This technique enhances spheroid sphericity, consistency, and yield for both single and mixed cell types.

More Related Videos

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device
06:39

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Published on: October 6, 2022

2.5K
Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
08:43

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

1.9K

Related Experiment Videos

Last Updated: Feb 20, 2026

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids
10:27

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Published on: November 7, 2019

6.9K
Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device
06:39

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Published on: October 6, 2022

2.5K
Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
08:43

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

1.9K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Cells in living tissue reside in complex 3D microenvironments.
  • Existing spheroid formation methods face challenges in uniformity, reproducibility, scalability, and throughput.
  • Accurate modeling of cellular environments is crucial for biological research.

Purpose of the Study:

  • To introduce a novel centrifugal microfluidic-based spheroid (CMS) formation method.
  • To generate highly controlled mono-culture and co-culture 3D spheroids.
  • To overcome limitations of existing spheroid generation techniques.

Main Methods:

  • Utilized a centrifugal microfluidic platform with circularly arrayed microwells.
  • Introduced cells to the center of a rotating platform for even distribution.
  • Applied centrifugal forces to create uniform hypergravity conditions in each microwell.

Main Results:

  • Achieved significantly improved sphericity and consistency in spheroid size and shape compared to conventional methods.
  • Demonstrated high production yields for both mono-culture and co-culture spheroids.
  • Successfully generated various co-culture spheroid configurations (concentric, Janus, sandwich) using specific cell types.

Conclusions:

  • The CMS formation method offers a versatile and controlled approach for generating 3D spheroids.
  • This technique represents a significant advancement in 3D cell culture technology.
  • The method provides a novel platform for studying cellular responses, including cell motility, under hypergravity conditions.