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Purification of fimbriae from Pasteurella haemolytica A-1
A A Potter1, K Ready, J Gilchrist
1Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada.
Microbial Pathogenesis
|April 1, 1988
Abstract:
Pasteurella haemolytica A-1 produces large, rigid fimbriae with a diameter of 12 nm and a denisty of 1.32. Their subunit molecular weight was 35,000 as determined by SDS-polyacrylamide gel electrophoresis and immunoblotting with monoclonal antibodies raised against native fimbriae. The isoelectric point of the purified fimbriae was 4.8. While Pasteurella haemolytica whole cells plus a crude shear fraction were capable of agglutinating bovine erythrocytes, purified fimbriae exhibited no such activity.