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Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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Related Experiment Video

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Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples
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Published on: November 23, 2016

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An Updated Protocol for High Throughput Plant Tissue Sectioning.

Jonathan A Atkinson1,2, Darren M Wells1

  • 1The Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Nottingham, United Kingdom.

Frontiers in Plant Science
|October 20, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces a 3D-printed mold method for rapid, high-quality plant tissue sectioning. This technique enhances throughput for plant anatomy studies and genetic screens, revealing significant root structural differences in wheat.

Keywords:
3D printingconfocal microscopycross sectionroot anatomytissue sectioning

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Area of Science:

  • Plant Biology
  • Plant Anatomy
  • Biotechnology

Background:

  • Accurate quantification of plant tissue and cellular structure is crucial for plant science research.
  • Existing sectioning methods are often low-throughput or require extensive practice.

Purpose of the Study:

  • To develop a rapid and high-quality method for plant tissue cross-sectioning.
  • To increase the throughput of traditional agarose embedding and sectioning techniques.
  • To provide a versatile method applicable to various plant tissues, including roots, leaves, and stems.

Main Methods:

  • Utilized custom-designed 3D-printed molds for embedding 5-15 plant roots in a single block.
  • Employed agarose embedding and sectioning for high-throughput processing.
  • Used a single fluorescent stain and laser scanning confocal microscopy for high-quality imaging of thick sections.
  • Provided CAD files for accessible 3D printing of embedding molds.

Main Results:

  • Demonstrated successful application to root, leaf, and stem tissues.
  • Achieved rapid, high-quality cross-sections suitable for detailed analysis.
  • Phenotyped wheat (Triticum aestivum) parent lines, identifying significant differences in adventitious cross-section area, stele area, xylem, phloem, metaxylem, and cortical cell file count.

Conclusions:

  • The 3D-printed mold method offers a significant improvement in throughput and quality for plant tissue sectioning.
  • This technique is valuable for forward genetic screens and phenotyping anatomical differences.
  • The method is adaptable for various plant tissues, facilitating broader research applications in plant science.