Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A
Àngela Ribes1,2,3, Sara Santiago-Felipe1,2,3, Andrea Bernardos1,2,4
1Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Universitat Politècnica de València Universitat de València Camino de Vera s/n 46022 Valencia Spain.
Chemistryopen
|October 20, 2017
Summary
Researchers developed novel aptamer-capped mesoporous silica materials for sensitive ochratoxin A (OTA) detection. These aptasensors show high selectivity and potential for real-world food safety applications.
Area of Science:
- Nanomaterials Science
- Analytical Chemistry
- Biotechnology
Background:
- Aptamers offer high affinity and selectivity for molecular recognition.
- Mesoporous nanomaterials show promise for advanced sensing applications.
Purpose of the Study:
- To develop aptamer-capped mesoporous silica materials for selective ochratoxin A (OTA) detection.
- To investigate covalent and electrostatic approaches for aptamer immobilization.
Main Methods:
- Loading rhodamine B into mesoporous silica nanoparticles.
- Capping nanoparticle pores with OTA-specific aptamers via covalent or electrostatic methods.
- Assessing dye release in the presence of OTA.
Main Results:
- The aptamer-capped materials selectively released dye upon OTA detection.
- Achieved high sensitivity with a limit of detection between 0.5-0.05 nM.
- Demonstrated excellent reproducibility, selectivity, and applicability in food matrices.
Conclusions:
- The developed aptasensors are effective for sensitive and selective OTA detection.
- The materials show significant potential for practical applications in food safety analysis.
