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GSK-J4-Mediated Transcriptomic Alterations in Differentiating Embryoid Bodies.

Chanchal Mandal1, Sun Hwa Kim1, Sung Chul Kang1

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|October 20, 2017
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Histone-modifying enzymes regulate cell differentiation. GSK-J4 treatment inhibited cellular proliferation and induced cell death, impacting neural differentiation by altering gene expression.

Keywords:
RNA sequencingcell cycle progressiongene expressionhistone demethylase enzyme

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Area of Science:

  • Cell Biology
  • Epigenetics
  • Developmental Biology

Background:

  • Histone-modifying enzymes are crucial for cellular differentiation.
  • Understanding their targets is key to controlling differentiation processes.

Purpose of the Study:

  • To profile target genes regulated by GSK-J4 during neural differentiation.
  • To investigate the effects of GSK-J4 on gene expression and cellular processes.

Main Methods:

  • Neural differentiation induced using retinoic acid in embryoid bodies.
  • RNA sequencing performed on GSK-J4 treated and untreated cells.
  • Bioinformatic analysis for gene expression and motif enrichment.

Main Results:

  • GSK-J4 exposure led to significant up- and down-regulation of 47 and 58 genes, respectively.
  • Differentially expressed genes were associated with suppressed proliferation, cell cycle arrest, and apoptosis.
  • Enrichment of specific motifs and identification of upstream regulators were observed.

Conclusions:

  • GSK-J4 inhibits cellular proliferation and induces cell death by affecting cell cycle progression.
  • These findings enhance understanding of histone-modifying enzyme roles in neural differentiation.
  • Further research is warranted to explore the detailed mechanisms involved.