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Observation of the influences of diosgenin on aging ovarian reserve and function in a mouse model
Mingjie Shen1, Cong Qi2, Yan-Ping Kuang3
1Department of Gynecology and Obstetrics, Shu Guang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Road Zhangheng No. 528, Pu Dong District, Shanghai, 201203, China.
Background:
The aim of this study was to investigate the impact of diosgenin, an important monomer of sapogenins in yams, on ovarian reserve in a natural aging mice model.
Study Design:
This randomized controlled trial included 60 9-month-old C57 naturally aging female mice. Twenty-one mice were assigned to the dio group and were fed a single dose of diosgenin (200 mg/kg/day) suspended in 0.3% CMC. Twenty mice were assigned to the DHEA group and were fed a single dose of DHEA (1.25 mg/kg/day) suspended in 0.3% CMC. The remaining 20 mice were assigned to the old control group and were fed a single dose of 0.3% CMC. Three months later, the reproductive performance of these female mice was determined by evaluating ovarian follicles and oocyte number and quality in IVF and comparing age-matched and young controls. The impact of NOBOX, GDF9 and BMP15 mRNA expression was also evaluated.
Results:
Diosgenin improves ovarian reserve in naturally aging mice in terms of increasing the number of primary follicles (P < 0.05) and serum levels of AMH (P < 0.05).
Conclusions:
Diosgenin could counteract age-associated ovarian dysfunction by improving the ovarian reserve in a natural aging mice model.
Insights
Diosgenin, a yam compound, improved ovarian reserve in aging mice by increasing follicle count and AMH levels. This suggests diosgenin can counteract age-related ovarian dysfunction.
Area of Science:
- Reproductive biology
- Gerontology
- Pharmacology
Background:
- Aging leads to diminished ovarian reserve and reproductive capacity.
- Diosgenin, a yam-derived sapogenin, is explored for its potential health benefits.
- Natural aging mouse models are crucial for studying age-related reproductive decline.
Purpose of the Study:
- To investigate the effects of diosgenin on ovarian reserve in naturally aging female mice.
- To evaluate diosgenin's impact on reproductive performance and key ovarian markers.
- To compare diosgenin's efficacy with dehydroepiandrosterone (DHEA) in an aging model.
Main Methods:
- A randomized controlled trial involving 60 naturally aging female mice (C57, 9 months old).
- Mice were allocated to three groups: diosgenin (200 mg/kg/day), DHEA (1.25 mg/kg/day), or control (0.3% CMC).
- Evaluations included ovarian follicle count, oocyte quality via IVF, and mRNA expression of NOBOX, GDF9, and BMP15 after 3 months.
Main Results:
- Diosgenin significantly increased the number of primary follicles compared to controls (P < 0.05).
- Serum levels of anti-Müllerian hormone (AMH) were significantly elevated in the diosgenin group (P < 0.05).
- Diosgenin demonstrated improvements in key indicators of ovarian reserve.
Conclusions:
- Diosgenin effectively counteracts age-associated ovarian dysfunction in a natural aging mouse model.
- The compound enhances ovarian reserve by improving follicle quantity and AMH levels.
- Diosgenin presents a potential therapeutic strategy for age-related reproductive decline.
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