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Updated: Feb 20, 2026

Split Point Analysis and Uncertainty Quantification of Thermal-Optical Organic/Elemental Carbon Measurements
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Uncertainty budgets for liquid waveguide CDOM absorption measurements.

Ina Lefering, Rüdiger Röttgers, Christian Utschig

    Applied Optics
    |October 20, 2017
    PubMed
    Summary
    This summary is machine-generated.

    Long path length liquid waveguide capillary cell (LWCC) systems offer enhanced sensitivity for measuring colored dissolved organic matter (CDOM) absorption. This study quantifies LWCC system uncertainties, revealing wavelength dependency and improving accuracy with stable sample pumping.

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    Area of Science:

    • Environmental Science
    • Analytical Chemistry
    • Spectroscopy

    Background:

    • Colored dissolved organic matter (CDOM) significantly impacts aquatic ecosystems and light penetration.
    • Long path length liquid waveguide capillary cell (LWCC) systems offer high sensitivity for CDOM spectral absorption measurements.
    • Limited data exists on the measurement uncertainties associated with LWCC systems.

    Purpose of the Study:

    • To quantify measurement precision and accuracy of LWCC systems with varying path lengths and cladding materials.
    • To assess the impact of sample handling (pumping vs. stationary) on measurement performance.
    • To evaluate the reliability of CDOM absorption data obtained from LWCC systems.

    Main Methods:

    • Cross-comparison of three LWCC systems (50, 100, 250 cm path lengths) with different cladding materials.
    • Quantification of measurement precision and accuracy across different wavelengths.
    • Evaluation of systematic artifacts, including salinity correction performance.
    • Null correction at 700 nm followed by CDOM absorption assessment at 440 nm.

    Main Results:

    • Measurement precision and accuracy exhibit strong wavelength dependency.
    • Stable sample pumping in LWCC systems improves measurement precision compared to stationary samples.
    • LWCC systems with higher refractive index cladding showed artifacts, while a 250 cm system with lower refractive index cladding showed promising results.
    • CDOM absorption data at 440 nm agreed within 5% root mean square percentage error after null correction.

    Conclusions:

    • LWCC systems provide sensitive measurements of CDOM spectral absorption.
    • System design, particularly cladding refractive index and path length, influences accuracy and introduces potential artifacts.
    • Further optimization of LWCC systems, especially salinity correction, is needed for robust CDOM quantification.