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Related Experiment Videos

H2O2 activates CD11b/CD18-dependent cell adhesion.

G Skoglund1, I Cotgreave, J Rincon

  • 1Department of Physiological Chemistry, Karolinska Institute, Stockholm, Sweden.

Biochemical and Biophysical Research Communications
|December 15, 1988
PubMed
Summary
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Hydrogen peroxide (H2O2) rapidly induces U-937 cell adhesion, mimicking phorbol ester effects. This suggests H2O2 may physiologically modulate leucocyte adhesion via protein kinase C activation.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Leukocyte adhesion is crucial for immune responses.
  • U-937 cells are a human monoblastoid cell line used to study cellular behavior.
  • Phorbol esters are known activators of protein kinase C and modulators of cell adhesion.

Purpose of the Study:

  • To investigate the effect of hydrogen peroxide (H2O2) on U-937 cell adhesion.
  • To compare the H2O2-induced adhesion mechanism with that of phorbol esters.
  • To explore the potential role of H2O2 as a physiological regulator of leukocyte adhesion.

Main Methods:

  • Treatment of U-937 cells with varying concentrations of H2O2.
  • Assessment of cell adhesion to plastic.
  • Analysis of time dependency, requirement for extracellular Mg2+, and inhibition by cytochalasin B.

Related Experiment Videos

  • Inhibition studies using monoclonal antibodies against CD11b and CD18.
  • Phosphorylation analysis of cellular substrates using 32P-incorporation.

    • Main Results:

    • Low concentrations of H2O2 rapidly induced U-937 cell adhesion (t1/2 ≈ 6 min).
    • Optimal stimulation occurred at 100 μM H2O2 (ED50 ≈ 50 μM).
    • H2O2-induced adhesion shared similarities with phorbol ester-induced adhesion, including Mg2+ dependence and inhibition by cytochalasin B and anti-CD11b/CD18 antibodies.
    • H2O2 treatment increased phosphorylation of pp28 and pp43, similar to phorbol ester effects.

    Conclusions:

    • Hydrogen peroxide acts as a potent inducer of U-937 cell adhesion.
    • The mechanism of H2O2-induced adhesion involves pathways similar to those activated by phorbol esters, potentially involving protein kinase C.
    • H2O2 may function as a physiological modulator of leukocyte adhesion.