Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

3.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.4K
Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

1.6K
Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
1.6K
Neural Regulation01:37

Neural Regulation

43.6K
Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
43.6K
Cholinergic Neurons: Neurotransmission01:23

Cholinergic Neurons: Neurotransmission

5.6K
Cholinergic neurotransmission involves the synthesis and the release of acetylcholine (ACh) in order to transmit nerve impulses across the synapse. The process begins with the synthesis of acetyl CoA, a precursor for ACh, from ATP, acetate, and coenzyme A in the mitochondria. Choline, another vital precursor, is transported inside the neuron through choline transporters, including high-affinity choline transporter CHT1, low-affinity choline transporter CTL1, and lower-affinity choline...
5.6K
Neurochemical Transmission: Sites of Drug Action01:26

Neurochemical Transmission: Sites of Drug Action

3.7K
Neurochemical transmission, the conduction of electrical impulses between neurons mediated by neurotransmitters, plays a vital role in various physiological processes. Autonomic drugs exert their effects by modulating neurotransmission within the autonomic nervous system. For instance, drugs such as hemicholinium block the precursor uptake necessary for synthesizing acetylcholine, an essential autonomic neurotransmitter. Following synthesis, neurotransmitters are stored in vesicles. Metyrosine...
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Astrocytic lipid dysregulation as an early driver of neurodegeneration.

Nature reviews. Neurology·2026
Same author

CRYAB is compromised in pathologically affected oligodendrocytes in multiple system atrophy.

Brain pathology (Zurich, Switzerland)·2026
Same author

Reduced serum interleukin-2 associates with higher motor severity and along with CD4 T cell alterations may be an early event in isolated REM Sleep Behaviour Disorder.

Brain, behavior, & immunity - health·2026
Same author

Ultra-rare variants in LAMA2 are risk factors for frontotemporal dementia and motor neuron disease.

Human molecular genetics·2026
Same author

Location and mapping of the human rostromedial tegmental nucleus and associated midbrain inhibitory nuclei regulating dopamine neurons.

Brain structure & function·2026
Same author

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum.

FEBS open bio·2026

Related Experiment Video

Updated: Feb 20, 2026

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model
08:24

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model

Published on: November 25, 2022

2.7K

α-Synuclein Regulates Neuronal Cholesterol Efflux.

Jen-Hsiang T Hsiao1,2,3, Glenda M Halliday4,5,6, Woojin Scott Kim7,8,9

  • 1Brain and Mind Centre, Sydney Medical School, The University of Sydney, Sydney NSW 2050, Australia. tony.hsiao@sydney.edu.au.

Molecules (Basel, Switzerland)
|October 20, 2017
PubMed
Summary

Alpha-synuclein, a protein linked to Parkinson's disease, was found to significantly enhance cholesterol removal from neuronal cells. This discovery highlights its role in regulating neuronal cholesterol and suggests new therapeutic avenues.

Keywords:
ABCA1Parkinson’s diseaseapolipoproteinscholesterol effluxα-synuclein

More Related Videos

Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays
09:36

Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays

Published on: August 13, 2017

7.1K
Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation
09:16

Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation

Published on: June 26, 2018

8.1K

Related Experiment Videos

Last Updated: Feb 20, 2026

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model
08:24

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model

Published on: November 25, 2022

2.7K
Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays
09:36

Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays

Published on: August 13, 2017

7.1K
Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation
09:16

Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation

Published on: June 26, 2018

8.1K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Alpha-synuclein is central to understanding α-synucleinopathies, including Parkinson's disease (PD).
  • The precise physiological function of α-synuclein remains largely unknown.
  • Alpha-synuclein shares biophysical properties with lipid-binding proteins and binds cholesterol, suggesting a role in lipid metabolism.

Purpose of the Study:

  • To investigate the potential role of α-synuclein in mediating cholesterol efflux from neuronal cells.
  • To determine if α-synuclein can act as a lipid acceptor, facilitating cholesterol removal.

Main Methods:

  • SK-N-SH neuronal cells were loaded with fluorescently labeled cholesterol.
  • Exogenous α-synuclein was applied to the cells.
  • Cholesterol efflux was measured using a standard cholesterol efflux assay, assessing dose-dependence, time-dependence, saturation, and involvement of the ABCA1 transporter.

Main Results:

  • Alpha-synuclein significantly stimulated cholesterol efflux from neuronal cells in a dose- and time-dependent manner.
  • The cholesterol efflux process mediated by α-synuclein was found to be saturable at 1.0 µg/mL.
  • This efflux was dependent on the presence of the ATP-binding cassette transporter A1 (ABCA1) on the plasma membrane.

Conclusions:

  • This study reveals a novel function of α-synuclein in regulating neuronal cholesterol homeostasis.
  • Alpha-synuclein plays a critical role in the process of cholesterol efflux.
  • The findings identify potential therapeutic targets for managing cellular cholesterol levels, relevant to neurodegenerative diseases.