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[Accuracy analysis and comparison of different serological detection methods in syphilis]
1Department of Clinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China.
Zhonghua Yi Xue Za Zhi
|October 20, 2017
Summary
External quality assessment (EQA) of syphilis serologic screening in China showed method-specific accuracy improvements. The colloidal gold method and Treponema pallidum particle agglutination (TPPA) test demonstrated significant gains in qualified rates.
Area of Science:
- Clinical diagnostics
- Public health
- Infectious disease screening
Background:
- Syphilis serologic screening is crucial for primary healthcare.
- External quality assessment (EQA) is vital for maintaining diagnostic accuracy.
- Chinese primary health care institutions utilize various syphilis screening methods.
Purpose of the Study:
- To compare the accuracy of different syphilis serologic screening methods.
- To analyze EQA data from Chinese primary health care institutions (2011-2016).
- To evaluate the impact of EQA implementation on screening performance.
Main Methods:
- Review of EQA data from 2011 to 2016.
- Inclusion of five serum samples per EQA shipment.
- Analysis of results from six distinct syphilis serologic screening methods: ELISA, colloidal gold, TPPA, RPR, TRUST, and CLIA.
Main Results:
- Qualified rates for colloidal gold method improved from 67.78% to 98.89%.
- Qualified rates for Treponema pallidum particle agglutination (TPPA) improved from 27.27% to 100%.
- Positive coincidence rate for TPPA increased to 100%, and negative coincidence rate for TRUST reached 100%.
Conclusions:
- Significant differences exist in the accuracy of various syphilis serological detection methods.
- EQA implementation led to improved qualified rates and coincidence rates in primary healthcare settings.
- Continuous EQA monitoring enhances syphilis screening accuracy in maternal and child health institutions.
