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DNA restriction fragment length polymorphisms at either end of the c-raf-1 locus at 3p25
S L Graziano1, G E Mark, C Murray
1Department of Medicine, SUNY-Health Science Center, Syracuse 13210.
Oncogene Research
|January 1, 1988
Abstract:
We report the identification and characterization of two restriction fragment polymorphisms (RFLPs) associated with the human c-raf-1 protooncogene located on chromosome 3 (3p25). EcoRI digestion produces an RFLP derived from the 5'flanking sequences with a heterozygosity of 28%, and TaqI produces an RFLP representing the 3' flanking sequences with a heterozygosity of 25%. The finding of RFLPs associated with a potential oncogene may provide a method of analysis for determining genetic susceptibilities to certain cancers.