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Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics

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Published on: May 3, 2024

Whole transcriptome analysis identifies differentially regulated networks between osteosarcoma and normal bone

Xuan Dung Ho^1,2, Phuong Phung¹, Van Q Le³

¹1 Department of Oncology, 155407 College of Medicine and Pharmacy , Hue University, Hue 53000, Vietnam.

Experimental Biology and Medicine (Maywood, N.J.)

|October 21, 2017

Summary

This summary is machine-generated.

Whole transcriptome analysis revealed significant gene expression differences in osteosarcoma bone, highlighting extracellular matrix degradation and collagen biosynthesis pathways. Chemotherapy may influence these pathways, impacting bone formation in osteosarcoma patients.

Keywords:

Osteosarcoma RNA sequencing gene expression profiling high-through put nucleotide sequencing osteogenic sarcoma transcriptome

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Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

Area of Science:

Oncology
Genomics
Molecular Biology

Background:

Osteosarcoma is a rare bone cancer with limited treatment options.
Understanding the molecular mechanisms of osteosarcoma is crucial for developing new therapies.

Purpose of the Study:

To identify differentially expressed genes in osteosarcoma bone tissue compared to normal bone.
To investigate the role of extracellular matrix (ECM) remodeling and collagen biosynthesis in osteosarcoma.
To explore the impact of chemotherapy on gene expression in osteosarcoma.

Main Methods:

Whole transcriptome RNA sequencing of 36 paired fresh-frozen osteosarcoma and non-tumoral bone samples.
Independent gene expression analysis of formalin-fixed paraffin-embedded samples.
Differential gene expression analysis using DESeq2 and edgeR.
Pathway analysis using Reactome.

Main Results:

Identified 5365 differentially expressed genes between osteosarcoma and normal bone (FDR < 0.05), with 3399 upregulated and 1966 downregulated.
Key genes like BTNL9, MMP14, and COL11A1 showed significant differential expression.
Pathway analysis revealed significant alterations in ECM degradation and collagen biosynthesis pathways.
Chemotherapy appeared to induce modifications in ECM and collagen biosynthesis.

Conclusions:

Alterations in extracellular matrix degradation are a key mechanism in osteosarcoma.
Chemotherapy may induce bone formation-related genes, suggesting a potential therapeutic effect on bone remodeling.