Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

16.8K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
16.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Traveler-derived importation risk underestimates regional disease activity: Evidence from Okinawa, Japan.

Journal of infection and public health·2026
Same author

Noise Management Preferences During Long-Term Hearing Aid Usage and Their Relation to Audiologic Factors.

Trends in hearing·2026
Same author

Development and Clinical Implementation of Voice-, Probe-, Neck Movement-Synchronized Functional Laryngeal Ultrasonography.

Journal of voice : official journal of the Voice Foundation·2026
Same author

Mobile Application as a Digital Therapeutic for Chronic Tinnitus: A Randomized Clinical Trial.

JAMA otolaryngology-- head & neck surgery·2026
Same author

Development of Magnetic Microbeads Equipped with Temperature-Responsive Artificial Antibodies for Efficient Anticancer Drug Administration.

Analytical chemistry·2026
Same author

Secondary severe acute respiratory syndrome coronavirus 2 transmission from childcare workers versus teachers in school-associated screening events, Okinawa, Japan, January-March 2022.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2026

Related Experiment Video

Updated: Feb 20, 2026

Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage
07:13

Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage

Published on: February 10, 2023

2.9K

Time-controllable Nkcc1 knockdown replicates reversible hearing loss in postnatal mice.

Takahisa Watabe1, Ming Xu2, Miho Watanabe3

  • 1Department of Otolaryngology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.

Scientific Reports
|October 21, 2017
PubMed
Summary

Researchers developed a novel transgenic mouse model for studying reversible hearing loss. This model allows for controlled knockdown of the NKCC1 protein, crucial for cochlear function, enabling new insights into hearing impairment.

More Related Videos

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration
12:21

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration

Published on: November 26, 2015

18.5K
Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research
08:30

Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research

Published on: June 8, 2017

18.8K

Related Experiment Videos

Last Updated: Feb 20, 2026

Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage
07:13

Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage

Published on: February 10, 2023

2.9K
The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration
12:21

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration

Published on: November 26, 2015

18.5K
Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research
08:30

Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research

Published on: June 8, 2017

18.8K

Area of Science:

  • Genetics
  • Otolaryngology
  • Molecular Biology

Background:

  • Identifying protein effects on hearing loss is challenging due to the lack of suitable animal models for reversible gene knockdown.
  • The cochlear potassium-chloride cotransporter (NKCC1) plays a vital role in hearing.
  • Previous models lacked the ability to reversibly manipulate gene expression in the cochlea.

Purpose of the Study:

  • To develop a transgenic mouse model for studying the causal effects of NKCC1 on hearing loss.
  • To enable reversible knockdown of NKCC1 expression in the cochlea.
  • To investigate partially reversible hearing loss mechanisms.

Main Methods:

  • Development of the Actin-tTS::Nkcc1tetO/tetO transgenic mouse line.
  • Utilized a tetracycline-inducible system for reversible Nkcc1 gene knockdown.
  • Administered doxycycline to regulate Nkcc1 expression and assess hearing phenotypes.

Main Results:

  • Complete suppression of Nkcc1 expression (~90% downregulation) led to null phenotypes.
  • Partial suppression of Nkcc1 (~70% downregulation) resulted in less hearing loss and mild cochlear changes.
  • Doxycycline treatment reactivated Nkcc1 expression, partially reversing hearing loss, particularly at high frequencies.

Conclusions:

  • The Actin-tTS::Nkcc1tetO/tetO mouse line provides a valuable tool for studying variable hearing loss.
  • This model allows for the investigation of reversible gene function in the cochlea.
  • It facilitates research into the mechanisms underlying partially reversible hearing loss.