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Down-regulation of IFITM1 and its growth inhibitory role in cervical squamous cell carcinoma

Weinan Zheng1,2, Zhimin Zhao1,2, Xinan Yi3

  • 1Department of Biochemistry and Molecular Biology, Department of Human Anatomy and Histology and Embryology, Basic Medical Science of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030 Hubei China.

Cancer Cell International
|October 21, 2017
PubMed
Abstract

Insights

Interferon-induced transmembrane protein 1 (IFITM1) may suppress cervical cancer growth. Lower IFITM1 gene expression and higher promoter methylation were observed in cervical cancer tissues, suggesting a protective role.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Cervical cancer is a leading cause of mortality in women globally.
  • Interferon-induced transmembrane protein 1 (IFITM1) has known roles in antiviral defense, cell adhesion, and carcinogenesis.
  • The specific function of the IFITM1 gene in cervical squamous cell carcinoma remains largely uncharacterized.

Purpose of the Study:

  • To investigate the expression patterns of the IFITM1 gene in cervical squamous cell carcinoma.
  • To elucidate the functional role of IFITM1 in the proliferation, migration, and invasion of cervical cancer cells.

Main Methods:

  • Quantitative real-time RT-PCR and immunohistochemistry were used to assess IFITM1 mRNA and protein levels in tumor and adjacent normal tissues.
  • Methylation-specific PCR was employed to detect promoter methylation of the IFITM1 gene.
  • HeLa cells were transfected with an IFITM1 expression vector to evaluate its effects on cell proliferation, migration, invasion, and apoptosis.

Main Results:

  • IFITM1 protein expression was significantly different between chronic cervicitis and cervical carcinoma samples (P < 0.01).
  • IFITM1 mRNA levels were significantly lower in cervical cancer tissues compared to normal tissues (P < 0.05), alongside increased promoter methylation (P < 0.05).
  • Overexpression of IFITM1 in HeLa cells led to decreased cell migration and invasion, inhibited proliferation, and increased apoptosis.

Conclusions:

  • Reduced expression and increased promoter methylation of the IFITM1 gene are associated with cervical squamous cell carcinoma.
  • IFITM1 gene expression demonstrates a potential tumor-suppressive role by inhibiting proliferation, migration, and invasion in cervical cancer cells.
Keywords:
Cell proliferationCervical squamous cell carcinomaIFITM1 geneMigration and invasion

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