Measuring Dynamics of Histone Proteins by Photobleaching in Arabidopsis Roots

Histone proteins play an important role in determining chromatin structure and gene expression. Studies in several systems have established that histones are in continuous turnover within the chromatin. It is therefore important to quantitatively measure the binding dynamics of these proteins in vivo. Photobleaching-based approaches such as fluorescence recovery after photobleaching (FRAP) are advantageous in that they are applied to living cells at a single cell level. In this chapter, I provide a detailed experimental protocol on how to perform histone FRAP experiments in Arabidopsis thaliana roots and how to analyze the most important parameters.