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Technical Review: Microscopy and Image Processing Tools to Analyze Plant Chromatin: Practical Considerations.

Célia Baroux1, Veit Schubert2

  • 1Department of Plant and Microbial Biology, Zürich-Basel Plant Science Center, University of Zürich, Zollikerstrasse 107, 8008, Zürich, Switzerland. cbaroux@botinst.uzh.ch.

Methods in Molecular Biology (Clifton, N.J.)
|October 21, 2017
PubMed
Summary

This review guides researchers in selecting the best fluorescence microscopy techniques for nucleus and chromatin analysis. It covers common and super-resolution methods, offering practical recommendations for high-quality imaging and data interpretation.

Keywords:
Confocal microscopyDeconvolutionElectron microscopyImage processingImage segmentationLight sheet microscopyPlant chromatinResolutionSignal quantificationSuper-resolution microscopyWidefield microscopy

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Area of Science:

  • Cell Biology
  • Microscopy
  • Biophysics

Background:

  • In situ nucleus and chromatin analysis requires advanced microscopy.
  • Fluorescent probes and proteins are crucial for static and live imaging.
  • A wide array of imaging instruments presents challenges in instrument selection and application.

Purpose of the Study:

  • To provide an overview of common and super-resolution fluorescence microscopy systems for nuclear and chromatin studies.
  • To guide users in selecting appropriate imaging instruments based on specific research needs.
  • To offer recommendations for optimizing image acquisition and quantitative analysis.

Main Methods:

  • Review of common fluorescence microscopy systems.
  • Discussion of super-resolution microscopy principles and applications.
  • Analysis of image processing tools for data evaluation.

Main Results:

  • Comparison of different microscopy techniques for nucleus and chromatin imaging.
  • Identification of key considerations for instrument selection.
  • Recommendations for achieving high-quality, informative images.

Conclusions:

  • Super-resolution microscopy bridges the resolution gap between fluorescence and electron microscopy.
  • Informed instrument choice and data processing are essential for accurate nuclear and chromatin organization studies.
  • This review provides practical guidance for researchers utilizing microscopy in cell biology.