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Updated: Aug 16, 2026

In Vivo SiRNA Transfection and Gene Knockdown in Spinal Cord via Rapid Noninvasive Lumbar Intrathecal Injections in Mice
Published on: March 22, 2014
[Neurotropic effects of tranquilizers administered intrathecally]
Abstract:
The effects of intrathecally administered benzodiazepines diazepam, flurazepam and flunitrazepam on the pain thresholds in rats were studied by using chronically implanted catheters. Despite benzodiazepine-induced marked muscle relaxation and sedative effect, benzodiazepines produced no significant changes in the thresholds during tests of tail flick and paw compression whereas morphine was effective in both tests. Experiments with 14C-diazepam and 3H-flunitrazepam showed that after intrathecal administration these substances rapidly enter the brain. It was found that 10-15 minutes after intrathecal administration 3H-flunitrazepam concentration in the frontal parts of the brain is 1/4 of its concentration in the lumbar spinal cord. The data suggest that these benzodiazepines possess no antinociceptive activity at intrathecal administration to rats and that way of administration fails to prevent their action on the brain.
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