Related Experiment Video
Updated: Feb 20, 2026
A Modified Precipitation Method to Isolate Urinary Exosomes
Published on: January 16, 2015
Susceptibility biomarker detection in urine exfoliate DNA
Pieranna Chiarella1, Damiano Carbonari1, Pasquale Capone1
1INAIL Research - Department of Occupational & Environmental Medicine, Epidemiology & Hygiene. Via Fontana Candida 1 - 00078 Monte Porzio Catone (RM), Italy.
Aim:
The occupational biomonitoring of exposures to carcinogens is carried out by measuring dose (metabolites) and susceptibility biomarkers (gene polymorphisms) in two biological matrices: urine for metabolite detection and blood for genotyping. Blood is the most common substrate but has some disadvantages including: invasiveness of the harvesting technique; need of specialized staff and equipment; and high infection risk.
Methods & Results:
We propose our in-house approach using urine as single sample in 20 volunteers for simultaneous detection of dose and susceptibility biomarkers in order to verify efficacy and feasibility.
Conclusion:
Despite the low number of subjects, interindividual and gender variability in DNA yield, urine genomic DNA is a valuable source for gene polymorphism studies when blood samples are not available. [Formula: see text].
