Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epidemiology and antimicrobial resistance of Mycoplasma genitalium among female sex workers in China: a cross-sectional study.

Sexual health·2026
Same author

Spherical Nucleic Acid Stabilized Cage Type Three-Dimensional Electrochemiluminescence Probe for Sensitive and Amplification-Free Diagnosis of Infectious Diseases.

Exploration (Beijing, China)·2026
Same author

Microfluidic single-cell culture represents a versatile approach for tumor stem cell expansion and tumor organoid generation.

Lab on a chip·2026
Same author

Spatially Ordered Dual-Atom Nanozymes for Mimicking Substrate-Induced Conformational Locking in Natural Enzymes.

Advanced materials (Deerfield Beach, Fla.)·2025
Same author

Single-Cell-Derived Tumor Organoid (STO) arrays on a microfluidic chip for personalized drug screening to address heterogeneity-induced drug resistance in colorectal cancer.

Microsystems & nanoengineering·2025
Same author

Digital-to-Droplet Microfluidic Platform Powered Digital Isothermal Nucleic Acid Amplification for On-Demand Detection of Post-Transplantation Infection-Causing Viruses.

Analytical chemistry·2025

Related Experiment Video

Updated: Feb 20, 2026

Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis
12:41

Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis

Published on: December 23, 2022

5.8K

Orthogonal Screening of Anticancer Drugs Using an Open-Access Microfluidic Tissue Array System.

Dongguo Lin1,2,3, Peiwen Li1, Jinqiong Lin1

  • 1Department of Laboratory Medicine, Guangzhou First People's Hospital, Guangzhou Medical University , Guangzhou 510180, China.

Analytical Chemistry
|October 21, 2017
PubMed
Summary

This study introduces a flexible microfluidic tissue array system for efficient drug combination screening. The novel system utilizes a nanoporous membrane to mimic endothelial layers, enabling complex cell assays and saving significant testing resources.

More Related Videos

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
11:28

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

Published on: October 4, 2017

10.8K
Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array HMCA-based Plates
08:32

Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array HMCA-based Plates

Published on: October 25, 2018

9.6K

Related Experiment Videos

Last Updated: Feb 20, 2026

Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis
12:41

Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis

Published on: December 23, 2022

5.8K
3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
11:28

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

Published on: October 4, 2017

10.8K
Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array HMCA-based Plates
08:32

Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array HMCA-based Plates

Published on: October 25, 2018

9.6K

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Drug Discovery

Background:

  • Current microfluidic cell culture systems face challenges in flexible liquid handling for drug combination screening.
  • Developing adaptable systems is crucial for advancing high-throughput drug discovery and personalized medicine.

Purpose of the Study:

  • To present an open-access microfluidic tissue array system for flexible and efficient drug combination screening.
  • To demonstrate the system's capability for complex multistep cell assays, including long-term culture and drug treatment.

Main Methods:

  • A microfluidic chip design featuring a nanoporous membrane between cell culture and media reservoir layers.
  • Utilizing the membrane as a flow-stop valve for cell distribution and for diffusion-based media exchange mimicking endothelial layers.
  • Integration with a liquid-transferring platform for automated multistep operations and cell-viability testing.

Main Results:

  • Rapid construction of a 10 × 10 tissue array in 90 seconds, followed by schedule-dependent drug testing.
  • Morphological and immunohistochemical assays confirmed the in vivo fidelity of the generated tumor tissue.
  • The nanoporous membrane's endothelial-mimicking function was validated by reduced anticancer agent efficacy; significant resource savings (67%) were achieved in drug combination screening.

Conclusions:

  • The developed microfluidic tissue array system offers a flexible, user-friendly, and efficient platform for complex cell-based assays.
  • This system is ideal for high-throughput drug combination screening, potentially accelerating drug discovery and development.
  • The system's ability to mimic in vivo conditions and reduce experimental costs highlights its potential impact on pharmaceutical research.