Detection of Hydrogen Peroxide with Fluorescent Dyes

Hydrogen peroxide (H₂O₂) is a powerful effector of redox signaling. It is able to oxidize cysteine residues, metal ion centers, and lipids. Understanding H₂O₂-mediated signaling requires, to some extent, measurement of H₂O₂ level. Recent Advances: Chemically and genetically encoded fluorescent probes for the detection of H₂O₂ are currently the most sensitive and popular. Novel probes are constantly being developed, with the latest progress particular with boronates and genetically encoded probes.

All currently available probes display limitations in terms of sensitivity, local and temporal resolution, and specificity in the detection of low H₂O₂ concentrations. In this review, we discuss the power of fluorescent probes and the systems in which they have been successfully employed. Moreover, we recommend approaches for overcoming probe limitations and for the avoidance of artifacts.

Constant improvements will lead to the generation of probes that are not only more sensitive but also specifically tailored to individual cellular compartments. Antioxid. Redox Signal. 29, 585-602.