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Affinity Chromatography

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

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Related Experiment Video

Updated: Feb 20, 2026

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

Aptamer affinity ligands in protein chromatography.

Gérald Perret¹, Egisto Boschetti²

¹LFB Biotechnologies, 91940, Les Ulis, France.

|October 22, 2017

Summary

Single chain oligonucleotide ligands, or aptamers, are a promising alternative to antibodies for protein purification via affinity chromatography. These highly specific and stable ligands offer advantages for large-scale applications.

Keywords:

Affinity chromatography Aptamer ligands Oligonucleotides Protein purification

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Last Updated: Feb 20, 2026

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

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Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Area of Science:

Biochemistry
Chromatography
Molecular Biology

Background:

Affinity chromatography is a key technique for protein purification.
Traditional ligands like antibodies have limitations in stability and availability.
Aptamers offer a novel and advantageous alternative for ligand selection.

Purpose of the Study:

To review the role and potential of aptamers in affinity chromatography for protein purification.
To highlight aptamers as a competitive alternative to antibodies in immunopurification.
To discuss the historical development and future prospects of aptamer-based chromatography.

Main Methods:

Review of historical developments in affinity chromatography.
Focus on aptamer selection technologies from large libraries.
Description of chemical grafting approaches for aptamer immobilization.
Compilation and analysis of experimental results from published literature.

Main Results:

Aptamers demonstrate high specificity, availability, and stability under harsh chromatographic conditions.
Selection and grafting technologies are advancing for practical aptamer application.
Experimental data supports the applicability of aptamers for large-scale protein purification.

Conclusions:

Aptamers represent a significant advancement in affinity ligand technology.
Further developments are expected to drive widespread adoption of aptamer-based chromatography for preparative applications.