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Engineering Novel and Improved Biocatalysts by Cell Surface Display.

Mason R Smith1, Eshita Khera2, Fei Wen1

  • 1Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109, United States.

Industrial & Engineering Chemistry Research
|October 24, 2017
PubMed
Summary

Cell surface display presents enzymes outside cells, overcoming limitations of soluble enzymes. This technology, combined with directed evolution, engineers better biocatalysts for sustainable chemical production.

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Area of Science:

  • Biotechnology and Biocatalysis
  • Synthetic Biology

Background:

  • Enzymes offer selective, eco-friendly catalysis under mild conditions, presenting a sustainable alternative to traditional catalysts.
  • Current industrial biocatalysis often uses soluble intracellular enzymes, facing challenges in purification and substrate accessibility.
  • Cell surface display (CSD) overcomes these limitations by presenting enzymes extracellularly on microbial hosts.

Purpose of the Study:

  • To review the principles of cell surface display technology.
  • To discuss its application in engineering enzymes with improved catalytic properties.
  • To highlight the development of surface-engineered microbes as whole-cell biocatalysts.

Main Methods:

  • Introduction to the molecular and cellular mechanisms underlying cell surface display systems.
  • Discussion of directed evolution strategies employed with CSD for enzyme enhancement.
  • Overview of microbial host engineering for extracellular enzyme presentation.

Main Results:

  • Cell surface display facilitates enzyme immobilization, simplifying purification and enhancing stability.
  • Directed evolution coupled with CSD enables the generation of enzymes with superior activity, selectivity, and substrate range.
  • Surface-engineered microbes serve as efficient whole-cell biocatalysts, integrating enzyme production and catalysis.

Conclusions:

  • Cell surface display is a versatile platform for enzyme engineering and whole-cell biocatalyst development.
  • This technology advances sustainable and efficient chemical manufacturing.
  • Further research in CSD promises novel biocatalytic solutions for industrial applications.