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R/G editing in GluA2Rflop modulates the functional difference between GluA1 flip and flop variants in GluA1/2R
Wei Wen1, Chi-Yen Lin1, Li Niu2
1Department of Chemistry, and Center for Neuroscience Research, University at Albany, SUNY, Albany, New York, 12222, United States.
Scientific Reports
|October 24, 2017
Summary
RNA editing at the arginine/glycine site in GluA2 receptor subunits modulates channel properties, but only in flop isoforms, impacting fast excitatory transmission.
Area of Science:
- Neuroscience
- Molecular Biology
- Biophysics
Background:
- Alternative splicing and RNA editing in α-amino-3-hydroxy-5-methyl-4-isoxazole-propionate (AMPA) receptors generate functionally distinct variants.
- Previous studies indicated no functional difference between flip and flop variants of the GluA1 AMPA receptor subunit in homomeric channels.
Purpose of the Study:
- To investigate the kinetic properties of AMPA receptor channels with varying arginine/glycine (R/G) editing and flip/flop splicing.
- To determine the influence of R/G editing on GluA2 subunit-containing homomeric and complex AMPA receptor channels.
Main Methods:
- Utilized laser-pulse photolysis combined with whole-cell recording techniques.
- Measured channel opening rates and other kinetic properties of AMPA receptor channels.
- Examined channels with different R/G editing statuses and flip/flop splicing variants.
Main Results:
- R/G editing in the GluA2 subunit affects channel properties in both homomeric (GluA2Q) and complex (GluA2Q/2R, GluA1/2R) channels.
- The effect of R/G editing is specific to flop isoforms, accelerating channel opening and desensitization in GluA1/2R channels.
- R/G editing did not influence channel closing rates or EC50 values.
Conclusions:
- R/G editing in the GluA2R flop isoform acts as a regulatory mechanism.
- This editing modulates the function of native receptors containing GluA2R, which are crucial for fast excitatory synaptic transmission.
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