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iQuant™ Analyser: A rapid quantitative immunoassay reader.

Jayaraj Joseph, Jayaraman Kiruthi Vasan, Malay Shah

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
    |October 25, 2017
    PubMed
    Summary

    The iQuant® Analyser provides accurate, automated quantitative results for point-of-care lateral flow immunoassays (LFIA). This portable immunoassay reader ensures reliable testing for key markers like HbA1C, Vitamin D, and TSH with minimal user input.

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    Area of Science:

    • Biomedical Engineering
    • Analytical Chemistry
    • Clinical Diagnostics

    Background:

    • Accurate, reliable instruments are crucial for quantitative point-of-care testing using lateral flow immunoassays (LFIA).
    • Existing methods may require complex operation or external calibration, limiting their suitability for rapid, at-site diagnostics.

    Purpose of the Study:

    • To introduce the iQuant® Analyser, a novel immunoassay reader designed for quantitative LFIA.
    • To demonstrate the instrument's capability in accurately measuring key clinical markers at the point of care.

    Main Methods:

    • Utilizes a laser-based confocal optics system to capture and digitize fluorescent signals from LFIA kits.
    • Employs kit-specific calibration data embedded on the test kit for automated result computation.
    • Features an intuitive, icon-based interface for simplified operation.

    Main Results:

    • Excellent repeatability with coefficient of variation (CoV) for area ratios < 1%.
    • Good inter-instrument reproducibility with CoV < 4%.
    • Accurate HbA1C results from blood samples, with errors < 4% compared to international standards.

    Conclusions:

    • The iQuant® Analyser is a portable, user-friendly instrument for rapid, quantitative immunoassay testing.
    • Its modular design and automated features make it ideal for point-of-care applications.
    • The reader provides accurate and reproducible results for various clinical markers without external calibration chips.