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Related Experiment Videos

Spatial and temporal aspects of cell signalling.

M J Berridge1, P H Cobbold, K S Cuthbertson

  • 1AFRC Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, University of Cambridge, U.K.

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
|July 26, 1988
PubMed
Summary
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Calcium puffs are generic InsP(3)-activated elementary calcium signals and are downregulated by prolonged hormonal stimulation to inhibit cellular calcium responses.

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Nature reviews. Molecular cell biology·2001

Cell signaling exhibits spatial and temporal organization, with localized inositol lipid pools controlling calcium gradients. Oscillations in intracellular calcium, often induced by inositol lipid hydrolysis, may encode information.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Signaling Pathways

Background:

  • Cell signaling demonstrates remarkable spatial and temporal organization.
  • Cells contain discrete, hormone-sensitive inositol lipid pools crucial for signaling.
  • Phosphoinositide signaling systems are often localized, establishing gradients of intracellular messengers like calcium.

Purpose of the Study:

  • To explore the spatial and temporal organization of cell signaling, particularly the role of phosphoinositides and calcium.
  • To investigate the mechanisms and potential functions of spontaneous intracellular calcium oscillations.
  • To understand the relationship between phosphoinositide hydrolysis and calcium signaling dynamics.

Main Methods:

  • Measurement of intracellular messengers using advanced techniques.

Related Experiment Videos

  • Observation of localized phosphoinositide signaling in specific cell types (e.g., Xenopus oocytes, Limulus photoreceptors, eggs).
  • Analysis of spontaneous intracellular calcium oscillations and their induction by agonists.

    • Main Results:

    • Inositol lipid signaling is highly concentrated in certain cell regions, creating localized calcium gradients.
    • Hydrolysis of inositol lipids at sperm entry in eggs causes localized Ins(1,4,5)P3 and calcium increases.
    • Spontaneous calcium oscillations, varying in frequency and pattern, are observed in various cell types and can be induced by inositol lipid-modulating agonists.

    Conclusions:

    • The spatial and temporal organization of cell signaling, involving phosphoinositides and calcium, is critical.
    • Calcium oscillations, potentially regulated by cytoplasmic or receptor-controlled oscillators, represent a significant aspect of cell signaling.
    • The frequency of calcium oscillations may serve as an information encoding mechanism, with potential implications for signal fidelity.