Related Experiment Video
Updated: Feb 20, 2026
Author Spotlight: Analysis of Ovarian Anatomy in Migratory Insects to Overcome Experimental Challenges
Published on: July 14, 2023
Morphological, Morphometric, and Molecular Characterization of Intraspecific Variations within Indian Populations of
Rajan Salalia1, R K Walia2, Vishal Singh Somvanshi3
1All India Coordinated Rice Improvement Programme, Division of Plant Breeding & Genetics, Faculty of Agriculture, Sher-e-Kashmir University of Agriculture & Technology, Chatha, Jammu 180009, Jammu and Kashmir, India.
Abstract:
Fourteen populations of Meloidogyne graminicola were collected from different agroecological regions of India. Morphological and morphometrical comparisons were made for various nematode life stages. Three populations (Hisar, New Delhi, and Samastipur) were different from typical M. graminicola on the basis of the length of eggs; J2 length, a-value, hyaline tail portion; male length, distance up to excretory pore, spicule and gubernaculum lengths; female length and width, stylet length, distance up to excretory pore, EPST (distance of excretory pore from anterior end / stylet length [females]) ratio, and vulval length. Morphological and morphometrical comparison with closely related species M. graminis, M. oryzae, M. salasi, M. triticoryzae, and M. lini clustered these populations into two groups: Anand, Bhubaneswar, Hyderabad, Jammu, Jorhat, Kalyani, Kanpur, Ludhiana, Mandya, Palampur, Vellayani grouped with M. graminicola, M. triticoryzae and M. salasi; whereas, Hisar, New Delhi, Samastipur grouped with M. oryzae and M. graminis. Molecular phylogenetic analysis using internal transcribed spacer (ITS) suggested that in spite of morphological differences, these populations belonged to M. graminicola.
