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Updated: Feb 20, 2026

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
Published on: October 4, 2017
Intramolecular glycosylation
Xiao G Jia1, Alexei V Demchenko1
1Department of Chemistry and Biochemistry, University of Missouri - St. Louis, One University Blvd., 434 Benton Hall (MC27), St. Louis, MO 63121, USA.
Abstract:
Carbohydrate oligomers remain challenging targets for chemists due to the requirement for elaborate protecting and leaving group manipulations, functionalization, tedious purification, and sophisticated characterization. Achieving high stereocontrol in glycosylation reactions is arguably the major hurdle that chemists experience. This review article overviews methods for intramolecular glycosylation reactions wherein the facial stereoselectivity is achieved by tethering of the glycosyl donor and acceptor counterparts.
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