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Behaviour of several enzymes of fetal chick intestine in tissue culture
1Institute of Pathological Biochemistry, Karl Marx University, Leipzig.
Abstract:
The behaviour of several enzymes is described of the fetal chick duodenum in tissue culture in a defined medium free of serum and hormones. During culture the activity of sucrase, maltase, alanine aminopeptidase, and gamma-glutamyltransferase is raised in tissue explants, whereas the activity of other enzymes (dipeptidyl peptidase IV, leucine amino-peptidase, alkaline phosphatase) remains constant. After culture, depending on the enzyme, a varying amount of activity is found in the medium, a part of which can be sedimented by ultracentrifugation. Sucrase is subject to the strongest increase in activity during culture and thus should represent a sensitive marker for investigating maturation processes in the fetal intestine and their disturbances.