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Multilocus molecular mapping of the mouse X chromosome
L J Mullins1, S G Grant, D A Stephenson
1Roswell Park Memorial Institute, Molecular and Cellular Biology Department, Buffalo, New York 14263.
Genomics
|October 1, 1988
Summary
Researchers mapped eight genes on the mouse X chromosome using restriction fragment length polymorphisms (RFLPs) and interspecies backcrosses. This mouse gene mapping provides insights into X chromosome evolution and human-mouse homology.
Area of Science:
- Genetics
- Genomics
- Comparative Genomics
Background:
- Gene mapping is crucial for understanding chromosome structure and evolution.
- Restriction fragment length polymorphisms (RFLPs) are valuable genetic markers for linkage analysis.
- Interspecies backcrosses facilitate the study of genetic variation between species.
Purpose of the Study:
- To assign three new genes to the mouse X chromosome.
- To map a total of eight genes spanning approximately 50 cM of the mouse X chromosome.
- To compare the mouse X chromosome with the human X chromosome and investigate evolutionary rearrangements.
Main Methods:
- Utilized restriction fragment length polymorphisms (RFLPs) and enzymatic variants.
- Performed interspecies backcrosses between C57BL/6JRos and Mus musculus/Mus spretus.
- Analyzed segregation of RFLPs in over 200 male progeny using Southern analysis.
Main Results:
- Successfully mapped eight genes, including Timp, Cf-8, and Rsvp, on the mouse X chromosome.
- Defined the gene order from the centromere as Otc, Timp, Hprt, Cf-9, (Cf-8/Rsvp/G6pd), Ags.
- Found no significant differences in map order or recombination frequency between different backcross series.
Conclusions:
- Established a framework for cumulative gene mapping on the mouse X chromosome.
- Provided insights into chromosomal rearrangements during mouse and human X chromosome evolution.
- Demonstrated the utility of functional gene probes for comparative mapping and evolutionary studies.