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Updated: Feb 20, 2026
Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs
Published on: May 24, 2016
Modeling of Lymphogenous and Hematogenous Metastasizing from Murine В16 Melanoma in Rats
O I Kit1, I V Kaplieva2, E M Frantsiyants1
1Rostov Research Institute of Oncology, Ministry of Health of the Russian Federation, Rostov-on-Don, Russia.
Abstract:
We developed a model of experimental melanoma. Intralienal xenogeneic transplantation of a suspension of melanoma cells B16 in physiological saline (0.1 ml; 1:10) was conducted to outbred male rats. In 6 months, histologically confirmed melanoma B16 in the spleen and its metastases in the liver, intestine, pancreas, adrenal glands, and lungs (hematogenous metastasis), as well as in the thymus and lymph nodes (lymphogenous metastasis) were revealed in rats. The proposed rat model of melanoma B16 metastasizes by the hematogenous and lymphogenous pathways, develops over 6 months, and allows receiving sufficient volume of material for analysis.
